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Figure 4: The association among HER2, ATF7, and cell junction in breast cancer. (a) Gene expression profiles of ATF7 and ERBB2 were obtained from the TCGA database. The association between ATF7 and ERBB2 in HER2-positive samples was determined using SPSS software (n = 79), (b) the HER2 recombinant plasmid was transfected into MCF-10A cells. The protein levels of ATF7, E-cadherin, and N-cadherin were determined by WB, (c) MCF-10A cells were transfected with the HER2-recombinant plasmid alone or with the combination of HER2- and ATF7-recombinant plasmids. Subsequently, the protein levels of ATF7, E-cadherin, and N-cadherin were determined by WB analysis. Relative quantitative analysis of protein expression is shown on the right. R = Pearson's correlation coefficient. ATF7 = Activating transcription factor 7, HER2 = Human epidermal growth factor receptor 2, WB = Western blot, ns = No significance, *,#P < 0.05, **,##P < 0.01

Figure 4: The association among HER2, <i>ATF7</i>, and cell junction in breast cancer. (a) Gene expression profiles of <i>ATF7</i> and <i>ERBB2</i> were obtained from the TCGA database. The association between <i>ATF7</i> and <i>ERBB2</i> in HER2-positive samples was determined using SPSS software (<i>n</i> = 79), (b) the HER2 recombinant plasmid was transfected into MCF-10A cells. The protein levels of <i>ATF7</i>, E-cadherin, and N-cadherin were determined by WB, (c) MCF-10A cells were transfected with the HER2-recombinant plasmid alone or with the combination of HER2- and <i>ATF7</i>-recombinant plasmids. Subsequently, the protein levels of <i>ATF7</i>, E-cadherin, and N-cadherin were determined by WB analysis. Relative quantitative analysis of protein expression is shown on the right. R = Pearson's correlation coefficient.<i> ATF7 </i>= Activating transcription factor 7, HER2 = Human epidermal growth factor receptor 2, WB = Western blot, ns = No significance, *,<sup>#</sup><i>P</i> < 0.05, **,<sup>##</sup><i>P</i> < 0.01