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Figure 1: ATF7 expression in breast cancer. (a) ATF7 expression in different molecular subtypes of breast cancer. *Normal basal-like versus luminal A, HER2-E or basal-like; #Luminal A versus HER2-E, (b) differential expression of ATF7 in HER2-negative and positive breast cancer samples as detected by IHC staining, (c) tissue microarray immunohistochemical staining of ATF7 in HER2-negative and positive breast cancer samples. Brown, ATF7-positive cells. Scale bar, 50 μm, (d) ATF7 and HER2 protein expression in MCF-10A normal mammary epithelial cells, MCF-7, and T47D cells, HER2-enriched SKBR3, and MDA-MB-453 cells as measured by WB analysis. Relative quantitative analysis of ATF7 protein expression is shown on the right, (e) transcriptional expression of ATF7 in MCF-10A, MCF-7, T47D, SKBR3, and MDA-MB-453 cells as measured by qRT-PCR. *HER2-enriched cell lines versus MCF-10A; #Luminal A subtype cells versus MCF-10A; @@ HER2-enriched cell lines versus luminal A subtype cells. **,##,@@P < 0.01. IHC = Immunohistochemistry, ATF7 = Activating transcription factor 7, HER2 = Human epidermal growth factor receptor 2, WB = Western blot, qRT-PCR = Quantitative real-time polymerase chain reaction

Figure 1: <i>ATF7</i> expression in breast cancer. (a) <i>ATF7</i> expression in different molecular subtypes of breast cancer. *Normal basal-like versus luminal A, HER2-E or basal-like; <sup>#</sup>Luminal A versus HER2-E, (b) differential expression of <i>ATF7</i> in HER2-negative and positive breast cancer samples as detected by IHC staining, (c) tissue microarray immunohistochemical staining of <i>ATF7</i> in HER2-negative and positive breast cancer samples. Brown, <i>ATF7</i>-positive cells. Scale bar, 50 μm, (d) <i>ATF7</i> and HER2 protein expression in MCF-10A normal mammary epithelial cells, MCF-7, and T47D cells, HER2-enriched SKBR3, and MDA-MB-453 cells as measured by WB analysis. Relative quantitative analysis of <i>ATF7</i> protein expression is shown on the right, (e) transcriptional expression of <i>ATF7</i> in MCF-10A, MCF-7, T47D, SKBR3, and MDA-MB-453 cells as measured by qRT-PCR. *HER2-enriched cell lines versus MCF-10A; <sup>#</sup>Luminal A subtype cells versus MCF-10A; <sup>@@</sup> HER2-enriched cell lines versus luminal A subtype cells. **<sup>,##,@@</sup><i>P</i> < 0.01. IHC = Immunohistochemistry, <i>ATF7 </i>= Activating transcription factor 7, HER2 = Human epidermal growth factor receptor 2, WB = Western blot, qRT-PCR = Quantitative real-time polymerase chain reaction