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Figure 3: (a) Immunocytochemistry of Bax and Bcl-2 in 4T1 cells treated with chlorogenic acid. Bax and Bcl-2 staining were analyzed by immunocytochemistry using confocal laser scanning microscopy. The nuclei stained with propidium iodide (observed red). Arrows show the primary antibodies of Bax or Bcl-2 (observed green). The merged images show clearly protein expression of Bax or Bcl-2 (×400). (b) Comparison of proteins expression percentage of Bax and Bcl-2 in 4T1 cells treated with chlorogenic acid (200 μM) and untreated 4T1 cells. Treated cells have significantly increased the proteins expression of Bax and decreased the proteins expression of Bcl-2 compared with the control. Each value is reported as mean ± SD of three experiments. Data were analyzed by independent samples t-test (***P < 0.001)

Figure 3: (a) Immunocytochemistry of Bax and Bcl-2 in 4T1 cells treated with chlorogenic acid. Bax and Bcl-2 staining were analyzed by immunocytochemistry using confocal laser scanning microscopy. The nuclei stained with propidium iodide (observed red). Arrows show the primary antibodies of Bax or Bcl-2 (observed green). The merged images show clearly protein expression of Bax or Bcl-2 (×400). (b) Comparison of proteins expression percentage of Bax and Bcl-2 in 4T1 cells treated with chlorogenic acid (200 μM) and untreated 4T1 cells. Treated cells have significantly increased the proteins expression of Bax and decreased the proteins expression of Bcl-2 compared with the control. Each value is reported as mean ± SD of three experiments. Data were analyzed by independent samples <i>t</i>-test (***<i>P</i> < 0.001)