Journal of Cancer Research and Therapeutics Close
 

Figure 2: (a) Chlorogenic acid's ability to inducing apoptosis in 4T1 cell lines. Flow cytometry dot plot of 4T1 cells stained with Annexin V-FITC and propidium iodide. A. Untreated cells used as controls and B. 4T1 cells treated by concentrations of 50, 100, 150, and 200 μM of chlorogenic acid for 24 h (left to right, respectively). Treated cells had significantly increased rates of early and late apoptosis compared with the control. All dot plots were drawn by the Tree Star FlowJo X v10.0.7 R2-win software, Becton, Dickinson & Company (BD), Boston, USA. (b) Apoptotic cells percentage, based on the evaluation of apoptosis by Annexin-V-propidium iodide. Comparison between early and late apoptosis is a result of different concentration of chlorogenic acid on 4T1 cells' effect. Each value was reported as mean ± SD of the mean of three experiments. Data analyzed by analysis of variance followed by Tukey's post hoc test (*P < 0.05, **P < 0.01)

Figure 2: (a) Chlorogenic acid's ability to inducing apoptosis in 4T1 cell lines. Flow cytometry dot plot of 4T1 cells stained with Annexin V-FITC and propidium iodide. A. Untreated cells used as controls and B. 4T1 cells treated by concentrations of 50, 100, 150, and 200 μM of chlorogenic acid for 24 h (left to right, respectively). Treated cells had significantly increased rates of early and late apoptosis compared with the control. All dot plots were drawn by the Tree Star FlowJo X v10.0.7 R2-win software, Becton, Dickinson & Company (BD), Boston, USA. (b) Apoptotic cells percentage, based on the evaluation of apoptosis by Annexin-V-propidium iodide. Comparison between early and late apoptosis is a result of different concentration of chlorogenic acid on 4T1 cells' effect. Each value was reported as mean ± SD of the mean of three experiments. Data analyzed by analysis of variance followed by Tukey's <i>post hoc</i> test (*<i>P</i> < 0.05, **<i>P</i> < 0.01)