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Figure 3: Metformin decreases specificity protein 1 expression in enhanced radiosensitivity of SMMC-7721 cells. (a) Quantitative reverse transcription polymerase chain reaction shows that metformin decreases specificity protein 1 mRNA levels of SMMC-7721 cells. (b) Western blot shows that metformin decreases specificity protein 1 protein levels. (c) Mithramycin A enhances the radiosensitivity of SMMC-7721 cells. Cells were treated with various doses of irradiation (0, 2, 4, 6, and 8 Gy) alone or irradiation with mithramycin A (100 nmol/L) for 24 h. *P < 0.05 versus control group, #P < 0.05 versus irradiation alone group

Figure 3: Metformin decreases specificity protein 1 expression in enhanced radiosensitivity of SMMC-7721 cells. (a) Quantitative reverse transcription polymerase chain reaction shows that metformin decreases specificity protein 1 mRNA levels of SMMC-7721 cells. (b) Western blot shows that metformin decreases specificity protein 1 protein levels. (c) Mithramycin A enhances the radiosensitivity of SMMC-7721 cells. Cells were treated with various doses of irradiation (0, 2, 4, 6, and 8 Gy) alone or irradiation with mithramycin A (100 nmol/L) for 24 h. *<i>P</i> < 0.05 versus control group, <sup>#</sup><i>P</i> < 0.05 versus irradiation alone group