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Figure 3: (a) Lactate dehydrogenase activity in the medium of U87MG cells treated with temozolomide and/or thymoquinone for 72 h. (b) Invasion assay using 24 well chambers. Invaded cells at randomly chosen fields were photographed after staining, and the number of invaded cells was quantified by measuring optical density. (c) Migration assay. Confluent cells were scratched and then treated with temozolomide and/or thymoquinone for 72 h. Closure of the scratches was photographed and calculated as a percentage of migration. (d) Adhesion assay, cells were pretreated with temozolomide and/or thymoquinone and seeded in a 4-well plate coated with gelatin. Attached cells were photographed after staining, and quantified by measuring optical density. P values were determined using one-way ANOVA (*P < 0.05; **P < 0.01 compared with control)

Figure 3: (a) Lactate dehydrogenase activity in the medium of U87MG cells treated with temozolomide and/or thymoquinone for 72 h. (b) Invasion assay using 24 well chambers. Invaded cells at randomly chosen fields were photographed after staining, and the number of invaded cells was quantified by measuring optical density. (c) Migration assay. Confluent cells were scratched and then treated with temozolomide and/or thymoquinone for 72 h. Closure of the scratches was photographed and calculated as a percentage of migration. (d) Adhesion assay, cells were pretreated with temozolomide and/or thymoquinone and seeded in a 4-well plate coated with gelatin. Attached cells were photographed after staining, and quantified by measuring optical density. <i>P</i> values were determined using one-way ANOVA (*<i>P</i> < 0.05; **<i>P</i> < 0.01 compared with control)