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Figure 1: Cigarette smoke condensate could promote BEAS-2B cell migration through neprilysin. BEAS-2B cell was culture and treated with cigarette smoke condensate (25 μg/ml) for 96 h and then subject to (a) MTT assay or (b) transwell assay (n = 5) (c) neprilysin shRNA also employed here to combine treated BEAS-2B cell with cigarette smoke condensate. Neprilysin protein amount was detected by western blots. (d) NEP protein level was normalized with GAPDH. Data were represent here as means ± standard error of mean (*P < 0.05, **P < 0.01, ***P < 0.001)

Figure 1: Cigarette smoke condensate could promote BEAS-2B cell migration through neprilysin. BEAS-2B cell was culture and treated with cigarette smoke condensate (25 μg/ml) for 96 h and then subject to (a) MTT assay or (b) transwell assay (<i>n</i> = 5) (c) neprilysin shRNA also employed here to combine treated BEAS-2B cell with cigarette smoke condensate. Neprilysin protein amount was detected by western blots. (d) NEP protein level was normalized with GAPDH. Data were represent here as means ± standard error of mean (*<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001)