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Figure 5: Effect of trichostatin A on mitochondrial membrane potential and apoptosis-inducing factor protein in Ca9.22 cells. (a) Mitochondrial membrane potential was measured using JC-1 assay kit and the data shown in the graph represent the mean ± standard deviation of triplicate experiments. *P < 0.05 compared with the DMSO treatment group (apoptosis inducing factor). (b) Apoptosis inducing factor protein expression was detected using Western blot analysis. COX4 and α-tubulin were used to normalize the mitochondrial and cytosolic protein, respectively. (c) The graph for cytosolic apoptosis inducing factor protein represents mean ± standard deviation of triplicate experiments, *P < 0.05 compared to the vehicle control group

Figure 5: Effect of trichostatin A on mitochondrial membrane potential and apoptosis-inducing factor protein in Ca9.22 cells. (a) Mitochondrial membrane potential was measured using JC-1 assay kit and the data shown in the graph represent the mean ± standard deviation of triplicate experiments. *<i>P</i> < 0.05 compared with the DMSO treatment group (apoptosis inducing factor). (b) Apoptosis inducing factor protein expression was detected using Western blot analysis. COX4 and <b>α</b>-tubulin were used to normalize the mitochondrial and cytosolic protein, respectively. (c) The graph for cytosolic apoptosis inducing factor protein represents mean ± standard deviation of triplicate experiments, *<i>P</i> < 0.05 compared to the vehicle control group