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Figure 4: Effect of trichostatin A on Bim and ERK1/2 pathway in HSC-3 cells. (a) HSC-3 cells were treated with DMSO or various concentrations of trichostatin A for 24 h. Proteins were extracted to analyze the expression levels of Bim by Western blot analysis. Protein levels were normalized to actin. The graph was the mean ± standard deviation of three independent experiments and significance (P < 0.05) compared with the control group was indicated (*). (b) Phospho-ERK and total-ERK expression were detected by Western blot analysis. (c) HSC-3 cells were co-treated with trichostatin A and AZD6244 for 24 h, and cell viability was evaluated by trypan blue exclusion assay (0.4%). The expression levels of phospho-ERK, Bim and cleaved PARP were analyzed by Western blot analysis

Figure 4: Effect of trichostatin A on Bim and ERK1/2 pathway in HSC-3 cells. (a) HSC-3 cells were treated with DMSO or various concentrations of trichostatin A for 24 h. Proteins were extracted to analyze the expression levels of Bim by Western blot analysis. Protein levels were normalized to actin. The graph was the mean ± standard deviation of three independent experiments and significance (<i>P</i> < 0.05) compared with the control group was indicated (*). (b) Phospho-ERK and total-ERK expression were detected by Western blot analysis. (c) HSC-3 cells were co-treated with trichostatin A and AZD6244 for 24 h, and cell viability was evaluated by trypan blue exclusion assay (0.4%). The expression levels of phospho-ERK, Bim and cleaved PARP were analyzed by Western blot analysis