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Figure 3: Effects of trichostatin A on caspase-dependent or independent apoptosis in oral squamous cell carcinoma cells. (a) Western blot analysis was performed to detect cleaved PARP and caspase 3. Actin was used to normalize the protein loading from each treatment. (b) HSC-3 and Ca9.22 cells were treated with trichostatin A (500 nM) for 24 h after 1 h pretreatment with Z-VAD (a pan caspase inhibitor, 2.5 μ M), followed by performing Western blot analysis

Figure 3: Effects of trichostatin A on caspase-dependent or independent apoptosis in oral squamous cell carcinoma cells. (a) Western blot analysis was performed to detect cleaved PARP and caspase 3. Actin was used to normalize the protein loading from each treatment. (b) HSC-3 and Ca9.22 cells were treated with trichostatin A (500 nM) for 24 h after 1 h pretreatment with Z-VAD (a pan caspase inhibitor, 2.5 <b>μ</b> M), followed by performing Western blot analysis