Figure 2: Effects of trichostatin A on the number of dead cells and morphological changes of oral squamous cell carcinoma cells. (a) Live (green) and dead (red) cells were determined by live/dead assay kit as mentioned in materials and methods (×200). (b) Fluorescence microscopy images of the 4',6-diamidino-2-phenylindole-stained in HSC-3 and Ca9.22 cells (×400). Dead cells or 4',6-diamidino-2-phenylindole-stained cells were counted, and the data shown in the graphs expressed the means ± standard deviation of triplicate experiments. *P < 0.05 compared with the DMSO treatment group; **P < 0.01 compared with the DMSO treatment group; ***P < 0.001 compared with the DMSO treatment group