Journal of Cancer Research and Therapeutics Close
 

Figure 1: Effect of trichostatin A on the viability and histone acetylation and of oral squamous cell carcinoma cells. (a) HSC-3 and Ca9.22 cells were treated with DMSO or different concentrations of trichostatin A (62.5, 125, 250, and 500 nM) for 24 h. The effects of trichostatin A on cell viability were analyzed by trypan blue exclusion assay (0.4%). The graph is mean ± standard deviation. *P < 0.05 compared with the DMSO treatment group; **P < 0.01 compared with the DMSO treatment group; ***P < 0.001 compared with the DMSO treatment group. (b) Histones were extracted using acid extraction and then analyzed by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis to detect acetylhistones. Equal amount of proteins were measured by Coomassie blue staining. The respective positions of all core histones are indicated on the left. SAHA-treated HSC-3 cells were used as a positive control. The data represent two independent experiments

Figure 1: Effect of trichostatin A on the viability and histone acetylation and of oral squamous cell carcinoma cells. (a) HSC-3 and Ca9.22 cells were treated with DMSO or different concentrations of trichostatin A (62.5, 125, 250, and 500 nM) for 24 h. The effects of trichostatin A on cell viability were analyzed by trypan blue exclusion assay (0.4%). The graph is mean ± standard deviation. *<i>P</i> < 0.05 compared with the DMSO treatment group; **<i>P</i> < 0.01 compared with the DMSO treatment group; ***<i>P</i> < 0.001 compared with the DMSO treatment group. (b) Histones were extracted using acid extraction and then analyzed by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis to detect acetylhistones. Equal amount of proteins were measured by Coomassie blue staining. The respective positions of all core histones are indicated on the left. SAHA-treated HSC-3 cells were used as a positive control. The data represent two independent experiments