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Figure 4: Second derivative Fourier transform infrared spectra of A549 cancer and clone cells. Mean ± standard deviation values of three experiments were represented for A549 cancer (square) and clones (circle) cells. Wave numbers specific for; (a) ADNA conformations/DNARNA hybrids at 970 and 1245 cm^{−1}, (b) ZDNA conformation at 1044 and 1067 cm^{−1}, (c) CO bond of carbohydrate at 1025 cm^{−1}, (d and e) imidazolemetal interactions at 1088 and 1560 cm^{−1}, (f) secondary structure of proteins at 1640, 1654, 1679 and 1696 cm^{−1}, (g and h) lipid at 1390, 1450, and 1390 cm^{−1}, (i) representative absorption spectrum of A549 clones and cancer cells of one experiment. *Significantly different at P< 0.05, **significantly different at P< 0.005. AU: Arbitrary unit 
