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Figure 4: Induction of apoptosis by lidamycin (LDM) in RPMI 8226 cells. Cells were treated with LDM for 48 h at the indicated concentrations. (a) Cells stained with DNA-binding dye, Hoechest 33342. Photographs were taken under ×400 fluorescent microscope. (b) Cells labeled with a combination of fluorescein isothiocyanate-Annexin V and propidium iodide and followed by flow cytometric analysis. The percentage of apoptotic cells (lower right quadrant) is indicated

Figure 4: Induction of apoptosis by lidamycin (LDM) in RPMI 8226 cells. Cells were treated with LDM for 48 h at the indicated concentrations. (a) Cells stained with DNA-binding dye, Hoechest 33342. Photographs were taken under ×400 fluorescent microscope. (b) Cells labeled with a combination of fluorescein isothiocyanate-Annexin V and propidium iodide and followed by flow cytometric analysis. The percentage of apoptotic cells (lower right quadrant) is indicated