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Figure 2: (a) CME-1 interferes migration ability of B16F10 cells. B16F10 cell monolayers at 90-95% confluence were serum starved for 24 h and then carefully wounded using sterilized pipette tips (t=0 h). After removing detached cells, they were incubated in RPMI1640 medium, with 250, 500 and 800 μg/ml CME-1 for up to 24 h at 37°C and photographed immediately (t=24 h); Original magnification: 40× (b) Representative bar diagram of migration assay is shown (Control cells after 24 h; Cells treated with 250, 500 and 800 μg/ml of CME-1 up to 24 h)

Figure 2: (a) CME-1 interferes migration ability of B16F10 cells. B16F10 cell monolayers at 90-95% confluence were serum starved for 24 h and then carefully wounded using sterilized pipette tips (t=0 h). After removing detached cells, they were incubated in RPMI1640 medium, with 250, 500 and 800 μg/ml CME-1 for up to 24 h at 37°C and photographed immediately (t=24 h); Original magnification: 40× (b) Representative bar diagram of migration assay is shown (Control cells after 24 h; Cells treated with 250, 500 and 800 μg/ml of CME-1 up to 24 h)