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Figure 1: Stably transfected MDR1-shRNAs cell clones and expressions of MDR1 and Pgy. Photos of top-panel observed under a fluorescent microscope (A-E) and bottom-panel observed under a light microscope (A1-E1) show the A2780 ovarian cancer cells stably transfected with the different constructs. A-A1: A2780 pSUPER-EGFP1 cells; B-B1: A2780 scrambled-shRNA cells; C-C1: A2780 MDR1- shRNA 1 cells; D-D1: A2780 MDR1-shRNA 2 cells. E-E1: A2780 MDR1- shRNA3 cells. Fig.1F represents the results of quantitative reverse transcriptase-PCR in the different stable transfectants and the changes in the expression of target gene MDR1 after downregulation of MDR1 in ovarian cancer cell lines. Significant difference is indicated by asterisk (***) for P < 0.001, which represents the A2780 mDR1-shRNA 2 cells compared with the A2780 cells or the A2780 pSUPER-EGFP1 cells or the A2780 scrambled shRNA cells, respectively. Fig.1G indicates the results of western blotting for detection of Pgy expression in the different transfected A2780 cells.

Figure 1: Stably transfected MDR1-shRNAs cell clones and expressions of MDR1 and Pgy. Photos of top-panel observed under a fluorescent microscope (A-E) and bottom-panel observed under a light microscope (A1-E1) show the A2780 ovarian cancer cells stably transfected with the different constructs. A-A1: A2780 pSUPER-EGFP1 cells; B-B1: A2780 scrambled-shRNA cells; C-C1: A2780 MDR1- shRNA 1 cells; D-D1: A2780 MDR1-shRNA 2 cells. E-E1: A2780 MDR1-
shRNA3 cells. Fig.1F represents the results of quantitative reverse transcriptase-PCR in the different stable transfectants and the changes in the expression of target gene MDR1 after downregulation of MDR1 in ovarian cancer cell lines. Significant difference is indicated by asterisk (***) for <i>P</i> < 0.001, which represents the A2780 mDR1-shRNA 2 cells compared with the A2780 cells or the A2780 pSUPER-EGFP1 cells or the A2780 scrambled shRNA cells, respectively. Fig.1G indicates the results of western blotting for detection of Pgy expression in the different transfected A2780 cells.