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Figure 3: Confirmation of differential expression of marker protein-coding genes and miRNAs by real time RT-PCR analysis. Y-axis denotes log 10 transformed RQs using GAPDH and RNU48 as housekeeping controls for protein-coding genes and miRNAs, respectively. Subtype A = blue, subtype B = green, subtype C = red, subtype D = pink. Black horizontal lines indicate median value for each cluster. Level of significance as determined by analysis of variance (ANOVA) for the differential expression of each gene/miRNA across the four molecular subtypes is also indicated

Figure 3: Confirmation of differential expression of marker protein-coding genes and miRNAs by real time RT-PCR analysis. Y-axis denotes log 10 transformed RQs using GAPDH and RNU48 as housekeeping controls for protein-coding genes and miRNAs, respectively. Subtype A = blue, subtype B = green, subtype C = red, subtype D = pink. Black horizontal lines indicate median value for each cluster. Level of significance as determined by analysis of variance (ANOVA) for the differential expression of each gene/miRNA across the four molecular subtypes is also indicated