Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 17  |  Issue : 2  |  Page : 393--400

Zinc oxide nanofluids: The influence of modality combinations on prostate cancer DU145 cells


Afsaneh Azhdari1, Razieh Jalal2 
1 Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Chemistry, Faculty of Science; Department of Research Cell and Molecular Biology, Institute of Biotechnology, Ferdowsi University of Mashhad, Iran

Correspondence Address:
Razieh Jalal
Azadi Square, Ferdowsi University of Mashhad, Mashhad, Razavi Khorasan Province
Iran

Aim: The combination of phototherapy and chemotherapy (chemophototherapy), presents a promising multimodal method for comprehensive cancer treatment. The aim of this study is to investigate the influence of low doses of zinc oxide (ZnO) nanofluids and ultraviolet A (UVA) irradiation on the cytotoxicity and cellular uptake of doxorubicin (DOX) on human prostate cancer DU145 cells. Materials and Methods: ZnO nanoparticles were prepared by the solvothermal method and 10% bovine serum albumin was used as the dispersant. The cytotoxic effect of DOX alone and in combination with different concentrations of ZnO nanofluids (0.95-15.6 μg/ml) in the presence and absence of UVA irradiation on DU145 cells was evaluated by -(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. DOX residue inside and outside of DU145 cells was explored by fluorescence microscopy and UV-Vis absorption spectroscopy, respectively. The role of ZnO nanofluids and UVA irradiation in DOX-induced apoptosis and cell cycle arrest were evaluated by DAPI staining, comet assay, and flow cytometry. Results: The results revealed that low dose of ZnO nanofluids (0.95 μg/ml) accompanied with irradiation enhanced the cytotoxicity and intracellular delivery of DOX in DU145 cells. The percentage of chromatin fragmentation/condensation and DNA tail of DU145 cells treated simultaneously with DOX and ZnO nanofluids was increased after UVA irradiation, whereas no significant changes in cell cycle progression were observed. Conclusion: The results indicate that ZnO nanofluids in the presence of UVA irradiation could increase DOX efficiency in DU145 cells, suggesting such modality combinations as a promising approach in cancer treatment.


How to cite this article:
Azhdari A, Jalal R. Zinc oxide nanofluids: The influence of modality combinations on prostate cancer DU145 cells.J Can Res Ther 2021;17:393-400


How to cite this URL:
Azhdari A, Jalal R. Zinc oxide nanofluids: The influence of modality combinations on prostate cancer DU145 cells. J Can Res Ther [serial online] 2021 [cited 2021 Oct 24 ];17:393-400
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2021;volume=17;issue=2;spage=393;epage=400;aulast=Azhdari;type=0