Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 16  |  Issue : 5  |  Page : 1112--1118

Insufficient radiofrequency ablation promotes epithelial–mesenchymal transition mediated by interleukin-6/signal transducer and activator of transcription 3/Snail pathway in the H22 cells


Tong Zhou1, Bin Liu1, Yongzheng Wang1, Wujie Wang1, Haiyang Chang1, Dong Li1, Yuliang Li1, Zhaomin Song2 
1 Department of Interventional Medicine, The Second Hospital of Shandong University; Interventional Oncology Institute of Shandong University, Jinan, Shandong Province, CN–250033, PR of China
2 Department of Oncology, The Third Hospital of Qinhuangdao, Qinhuangdao, Hebei Province, China

Correspondence Address:
Yuliang Li
Department of Interventional Medicine, The Second Hospital of Shandong University, 247 Beiyuan Road, Jinan 250033, Shandong Province; Interventional Oncology Institute of Shandong University, Jinan 250033, Shandong Province
PR of China
Zhaomin Song
Department of Oncology, The Third Hospital of Qinhuangdao, 222 Jianguo Road, Qinhuangdao, Hebei Province
China

Context: Radiofrequency ablation (RFA), an established and minimally invasive therapy for hepatocellular carcinoma, has become an important treatment strategy. However, tumor aggressiveness remains a common problem. The epithelial–mesenchymal transition (EMT) is thought to play an important role in this process. Design and Aims: Due to limited sample volumes harvested from patients, we established a heat-treated cell line and a mouse model to investigate the mechanisms of incomplete ablation in EMT. Materials and Methods: We heat-treated H22 and HepG2 cells using a water bath to determine a suitable temperature for incomplete RFA. Male BALB/c mice were orthotopically transplanted with H22 cells and then subjected to incomplete ablation. Changes in the EMT biomarkers were detected by real-time polymerase chain reaction, western blotting, and immunofluorescence. Statistical Analysis: The experimental results are expressed as means ± standard deviations. Results: Incomplete RFA promoted EMT, downregulated E-cadherin, upregulated vimentin and Snail, and enhanced the phosphorylation of signal transducer and activator of transcription 3 (STAT3) both in vivo and in vitro. Moreover, interleukin (IL)-6 secretion increased after heat treatment in the H22 cells. AG490, an IL-6 inhibitor, inhibited the occurrence of EMT. Conclusions: Insufficient ablation performed at low temperature successfully induces EMT and promotes tumor aggressiveness, which is mediated by the IL-6/STAT3/Snail pathway in both cell and mouse models.


How to cite this article:
Zhou T, Liu B, Wang Y, Wang W, Chang H, Li D, Li Y, Song Z. Insufficient radiofrequency ablation promotes epithelial–mesenchymal transition mediated by interleukin-6/signal transducer and activator of transcription 3/Snail pathway in the H22 cells.J Can Res Ther 2020;16:1112-1118


How to cite this URL:
Zhou T, Liu B, Wang Y, Wang W, Chang H, Li D, Li Y, Song Z. Insufficient radiofrequency ablation promotes epithelial–mesenchymal transition mediated by interleukin-6/signal transducer and activator of transcription 3/Snail pathway in the H22 cells. J Can Res Ther [serial online] 2020 [cited 2020 Nov 24 ];16:1112-1118
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2020;volume=16;issue=5;spage=1112;epage=1118;aulast=Zhou;type=0