Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 10  |  Page : 713--718

Overexpression of Tat-interacting protein 30 inhibits the proliferation, migration, invasion and promotes apoptosis in bladder cancer cells


Yeping Li1, Bing Cai1, Sansan Chen2, Xinyang Fu2, Xiang Pang2, Xiaojun Zhu2, Honggang Qi2, Wanlong Tan2 
1 Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515; Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang 325000, China
2 Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

Correspondence Address:
Wanlong Tan
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515
China

Aims: Tat-interacting protein 30 (TIP30), a transcriptional repressor, possesses antitumor effect in different cancer cells. However, little is known about the function of TIP30 in bladder cancer till now. Materials and Methods: A TIP30-overexpressing plasmid was transfected into the bladder cancer cells (T24). The cell cycle and apoptosis were detected by flow cytometry. The cell proliferation was analyzed using the cell counting kit-8 assay. The migrative and invasive abilities of T24 cells were measured by the transwell assay. The expression of TIP30, cell cycle proteins, migration-related proteins, and cell apoptosis-related proteins was assessed by Western blotting. Results: The cell proliferation, migration, and invasion of T24 cells were inhibited by overexpression of TIP30. Moreover, the rate of cell apoptosis was increased by the overexpression of TIP30. The expression of cell cycle proteins, phosphorylated EGFR, p-Akt, Bcl-2, cyclin D, cyclin E), migration-related proteins (matrix metalloproteinases 2 [MMP2], MMP6, MMP9), were downregulated, and cell apoptosis-related proteins (bax, cleaved caspase3) were upregulated. Conclusions: These results suggest that TIP30, as a tumor suppressor in the bladder cancer, might be served as a target in cancer therapies in the future.


How to cite this article:
Li Y, Cai B, Chen S, Fu X, Pang X, Zhu X, Qi H, Tan W. Overexpression of Tat-interacting protein 30 inhibits the proliferation, migration, invasion and promotes apoptosis in bladder cancer cells.J Can Res Ther 2018;14:713-718


How to cite this URL:
Li Y, Cai B, Chen S, Fu X, Pang X, Zhu X, Qi H, Tan W. Overexpression of Tat-interacting protein 30 inhibits the proliferation, migration, invasion and promotes apoptosis in bladder cancer cells. J Can Res Ther [serial online] 2018 [cited 2020 Nov 25 ];14:713-718
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2018;volume=14;issue=10;spage=713;epage=718;aulast=Li;type=0