Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 10  |  Page : 648--655

X-linked inhibitor of apoptosis protein inhibitor Embelin induces apoptosis via PI3K/Akt pathway and inhibits invasion in osteosarcoma cells


Hao Qian1, Tao Huang1, Yao Chen1, Xiucheng Li1, Weihua Gong2, Guangjian Jiang2, Wei Zhang1, Shuo Cheng1, Xuhui Li2, Pengcheng Li1 
1 Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China
2 Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, 02215 MA, USA

Correspondence Address:
Tao Huang
Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning
China

Background: Embelin is an active compound identified as a novel X-linked inhibitor of apoptosis protein (XIAP) inhibitor from the Embelia ribes that exhibits various medicinal effects including anti-inflammatory and anticancer activities. However, the therapeutic effect of Embelin to human osteosarcoma is not yet determined. Objectives: In this study, we evaluated the sensitizing potential of Embelin on promoting apoptosis to cause osteosarcoma cell death and inhibiting its invasion. Methods: We uesd 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to detect the survival rates of osteosarcoma cells, Western blot to detect the expression of proteins in U-2 OS and MG63 cells, and fluorescence microscope to observe the morphology of apoptotic cells. Results: The survival of osteosarcoma cells decreased, When Embelin was used. Obvious condensed and flared fluorescence was observed, when used high-dose Embelin. There was an increase of caspase-3, cleaved caspase-3, caspase-8, and caspase-9 in Embelin group, while PI3K, AKt, p-AKt, X-linked inhibitor of apoptosis protein, and MMP-9 were downregulated. The invasion of Embelin application was significantly lower than that of the control application. Conclusion: Embelin promoted apoptosis via XIAP and PI3K/Akt signaling pathway. XIAP inhibitor Embelin inducing apoptosis could cause osteosarcoma cell death and inhibit its invasion.


How to cite this article:
Qian H, Huang T, Chen Y, Li X, Gong W, Jiang G, Zhang W, Cheng S, Li X, Li P. X-linked inhibitor of apoptosis protein inhibitor Embelin induces apoptosis via PI3K/Akt pathway and inhibits invasion in osteosarcoma cells.J Can Res Ther 2018;14:648-655


How to cite this URL:
Qian H, Huang T, Chen Y, Li X, Gong W, Jiang G, Zhang W, Cheng S, Li X, Li P. X-linked inhibitor of apoptosis protein inhibitor Embelin induces apoptosis via PI3K/Akt pathway and inhibits invasion in osteosarcoma cells. J Can Res Ther [serial online] 2018 [cited 2022 Jun 26 ];14:648-655
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2018;volume=14;issue=10;spage=648;epage=655;aulast=Qian;type=0