Year : 2015 | Volume
: 11 | Issue : 6 | Page : 216--221
MicoRNA-451 is a novel tumor suppressor via targeting c-myc in head and neck squamous cell carcinomas
Huimin Wang, Guozheng Zhang, Zhiyan Wu, Baocai Lu, Dongjie Yuan, Xiao Li, Zhenmin Lu
Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Xinxiang Medical College, Xinxiang 453100, Henan Province, China
Objective: Head and neck squamous cell carcinoma (HNSCC) represents as a common malignancy with increasing incidence in the worldwide. The fact of its poor survival rate urgently requires developing efficient predictive biomarkers for clinical use. MicroRNAs (miRNAs) recently represent as a novel direction for early diagnosis and prognosis prediction in HNSCC therapy. In this study, we comprehensively investigated the function and putative target of miRNA-451 in vitro.
Methods: The expression of miRNA-451 was detected in HNSCC tissues and cell lines by real-time PCR. Forced expression or inhibition of miRNA-451 was done by transient transfection of mimics or inhibitor of miRNA-451 into indicated cells, respectively. Cell proliferation was evaluated by cell counting and crystal staining. Afterwards, we perform western blot to verify the expression of the miRNA-451 predicted target, c-myc, after miRNA-451 was overexpressed.
Results: We showed that miRNA-451 was downregulated in paired HNSCC tissues as well as in cell lines. And overexpression of miRNA-451 in cells with low endogenous expression of miRNA-451 accelerated proliferation. To the contrast, knockdown of miRNA-451 in cells with high levels of miRNA-451 significantly reduced cell growth rate. Furthermore, we used bioinformatics and cellular methods to predict and prove that c-myc was targeted by miRNA-451, since forced expression of miRNA-451 leaded to decreased c-myc protein expression in HNSCC cells.
Conclusion: Our findings identify miRNA-451 as a potential biomarker and suggest a key role of miRNA-451-c-myc pathway in HNSCC cell transformation, which could represent a novel therapeutic strategy in HNSCC treatment.
Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Xinxiang Medical College, Xinxiang 453100, Henan Province
|How to cite this article:|
Wang H, Zhang G, Wu Z, Lu B, Yuan D, Li X, Lu Z. MicoRNA-451 is a novel tumor suppressor via targeting c-myc in head and neck squamous cell carcinomas.J Can Res Ther 2015;11:216-221
|How to cite this URL:|
Wang H, Zhang G, Wu Z, Lu B, Yuan D, Li X, Lu Z. MicoRNA-451 is a novel tumor suppressor via targeting c-myc in head and neck squamous cell carcinomas. J Can Res Ther [serial online] 2015 [cited 2021 Oct 18 ];11:216-221
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2015;volume=11;issue=6;spage=216;epage=221;aulast=Wang;type=0