Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 10  |  Issue : 1  |  Page : 68--72

Comparison of nucleostemin gene expression in CD133+ and CD133− cell population in colon cancer cell line HT29


Noosha Zia-Jahromi1, Seyed Hossein Hejazi2, Mojtaba Panjepour3, Kazem Parivar1, Marjan Gharagozloo4 
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Biochemistry, School of Pharmacology, Isfahan University, Isfahan, Iran
4 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Correspondence Address:
Noosha Zia-Jahromi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran
Iran

Background: Nucleostemin has been shown to be essential for proliferation and survival of colon cancer cells. In this study, we evaluate and comparing nucleostemin expression in CD133+ and CD133- colon cancer cell line HT29. Materials and Methods: After preparation and culturing of HT29 cell line, isolation was performed using magnetic cell separation system by CD133 MicroBeads and phycoerythrin conjugated to monoclonal anti-human CD133 monoclonal antibody and analyzed with flow cytometry. For quantitative expression of nucleostemin in HT29, CD133+ and CD133- cells used specific nucleostemin primer and glyceraldehyde 3-phosphate dehydrogenase primer as endogenous control. Results: The results showed the percentage of CD133+ cells in HT29 colon cancer cell line ranged from 36.5% to 41.5%, whereas the percentage of CD133- cell ranged from 58.5% to 63.5%. The expression rate of nucleostemin in HT29, CD133+ and CD133- cells were 1.44 ± 0.78, 1.60 ± 0.70 and 1.00 ± 0.18 (respectively). The comparison of expression rate represents no significant difference in nucleostemin expression in CD133+, CD133- and HT29 colon cancer cells. Conclusion: It is concluded that nucleostemin expression could not be specific in a certain type of cells in colon cancer cell line HT29 and controlling strategies in colon cancer must not be focused on one certain type of colon cancer cells as main expressing nucleostemin gene.


How to cite this article:
Zia-Jahromi N, Hejazi SH, Panjepour M, Parivar K, Gharagozloo M. Comparison of nucleostemin gene expression in CD133+ and CD133− cell population in colon cancer cell line HT29.J Can Res Ther 2014;10:68-72


How to cite this URL:
Zia-Jahromi N, Hejazi SH, Panjepour M, Parivar K, Gharagozloo M. Comparison of nucleostemin gene expression in CD133+ and CD133− cell population in colon cancer cell line HT29. J Can Res Ther [serial online] 2014 [cited 2021 Sep 23 ];10:68-72
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2014;volume=10;issue=1;spage=68;epage=72;aulast=Zia-Jahromi;type=0