Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2012  |  Volume : 8  |  Issue : 3  |  Page : 399--403

Cell cycle analysis of the CD133 + and CD133 - cells isolated from human colorectal cancer


Marjan Gharagozloo1, Hamid R Mirzaei1, Bahram Bagherpour1, Abbas Rezaei1, Hamid Kalantari2, Mohammad H Sanei3, Mohsen Hosseini4, Gholamreza Mohajeri5, Abbas Tabatabai5, Mozaffar Hashemi5 
1 Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Gasteroentrology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Biostatistics and Epidemiology, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran
5 Department of Surgery, Alzahra University Hospital, Isfahan University of Medical Sciences, Isfahan, Iran

Correspondence Address:
Marjan Gharagozloo
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
Iran

Aim: The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133 + and CD133 - cells, isolated from primary human colorectal tumors. Materials and Methods: After mechanical and enzymatic dissociation of the tumor samples, CD133 + and CD133 - subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133 + and CD133 - cells isolated from primary and liver metastatic cancer cells. Results: The results indicated that CD133 + cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133 - cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133 - cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase. Conclusion: The present study provides evidence that CD133 + cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-therapy patients.


How to cite this article:
Gharagozloo M, Mirzaei HR, Bagherpour B, Rezaei A, Kalantari H, Sanei MH, Hosseini M, Mohajeri G, Tabatabai A, Hashemi M. Cell cycle analysis of the CD133 + and CD133 - cells isolated from human colorectal cancer.J Can Res Ther 2012;8:399-403


How to cite this URL:
Gharagozloo M, Mirzaei HR, Bagherpour B, Rezaei A, Kalantari H, Sanei MH, Hosseini M, Mohajeri G, Tabatabai A, Hashemi M. Cell cycle analysis of the CD133 + and CD133 - cells isolated from human colorectal cancer. J Can Res Ther [serial online] 2012 [cited 2022 Jan 21 ];8:399-403
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2012;volume=8;issue=3;spage=399;epage=403;aulast=Gharagozloo;type=0