Journal of Cancer Research and Therapeutics

BRIEF REPORT
Year
: 2007  |  Volume : 3  |  Issue : 2  |  Page : 124--126

Conventional and fluorescence in situ hybridization analysis of three-way complex BCR-ABL rearrangement in a chronic myeloid leukemia patient


Bani Bandana Ganguly1, Nitin N Kadam1, MB Agarwal2 
1 MGM Center for Genetic Research and Diagnosis, MGM’s New Bombay Hospital, Navi Mumbai, India
2 Hematology Centre, Dadar (E), Mumbai, India

Correspondence Address:
Bani Bandana Ganguly
MGM Center for Genetic Research and Diagnosis, MGM’s New Bombay Hospital, Vashi plot 35, Sector 3, Navi Mumbai - 400 703
India

Chromosomal analysis was carried out in bone marrow sample of an 11-year-old girl suspected of myeloproliferative disorder. Conventional G-banding study detected a complex three-way translocation involving 7, 9 and 22, which has resulted in the formation of a variant Philadelphia chromosome causing rearrangement of abl and bcr genes in 87% cells. Fluorescence in situ hybridization (FISH) confirmed the fusion of bcr-abl oncogene. Thus the bone marrow karyotype was observed as 46,XX (13%) / 46,XX,t(7;9;22)(q11;q34;q11) (87%). Hyperdiploidy was present in two cells. In this study, both conventional cytogenetic and FISH diagnosis proved to be significant to identify the variant nature of the Philadelphia chromosome and hyperdiploid condition for introduction of a suitable treatment regimen and estimation of life expectancy of the young girl.


How to cite this article:
Ganguly BB, Kadam NN, Agarwal M B. Conventional and fluorescence in situ hybridization analysis of three-way complex BCR-ABL rearrangement in a chronic myeloid leukemia patient.J Can Res Ther 2007;3:124-126


How to cite this URL:
Ganguly BB, Kadam NN, Agarwal M B. Conventional and fluorescence in situ hybridization analysis of three-way complex BCR-ABL rearrangement in a chronic myeloid leukemia patient. J Can Res Ther [serial online] 2007 [cited 2020 Dec 1 ];3:124-126
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2007;volume=3;issue=2;spage=124;epage=126;aulast=Ganguly;type=0