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Regulation of XPA could play a role in inhibition of radiation-induced bystander effects in QU-DB cells at high doses
Mohammad Taghi Bahreyni Toossi1, Hosein Azimian1, Shokouhozaman Soleymanifard2, Habibeh Vosoughi3, Elham Dolat3, Abdul Rahim Rezaei4, Sara Khademi5
1 Medical Physics Research Center; Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2 Medical Physics Research Center; Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences; Department of Medical Physics, Omid Hospital, Mashhad, Iran
3 Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4 Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5 Department of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
Correspondence Address:
Hosein Azimian
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad
Iran
Sara Khademi
Department of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jcrt.JCRT_503_18
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Introduction: Radiation-induced bystander effects (RIBE) is the radiobiological effects detected in nonirradiated cells that have received signals from neighboring irradiated cells. In some studies, there are observations that RIBE unexpectedly reduces at high doses. In this study, the expression of two selected apoptotic and repair genes and their possible role in the formation of this unexpected reduction is examined.
Materials and Methods: The QU-DB cells were irradiated with gamma rays of a60 Co teletherapy unit at doses of 2, 4, 6, and 8 Gy. One hour following irradiation, their culture media were transferred to bystander cells to induced RIBE. After 24 h incubation, the RNA of cells was isolated and cDNA synthesized. Expression levels of BAX, XPA, and XPA/BAX ratio were examined by relative quantitative reverse transcription-polymerase chain reaction.
Results: In target cells, up-regulation of both genes was observed at all doses. In bystander cells, at the low dose (2 Gy), the expression of BAX was more than XPA; at 4 Gy, the ratio was balanced. A significant correlation was found between the XPA/BAX ratio and the dose, at high doses pattern of gene expression dominated by DNA repair gene.
Conclusion: Gene expression profile was distinctive in bystander cells compared to target cells. The observed linear increasing of the ratio of XPA/BAX could support the hypothesis that the DNA repair system is stimulated and causes a reduction in RIBE at high doses.
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