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ORIGINAL ARTICLE
Year : 2016  |  Volume : 12  |  Issue : 5  |  Page : 1-4

Gemcitabine inhibits proliferation and induces apoptosis in human pancreatic cancer PANC-1 cells


1 Department of Oncology, Xinxiang Central Hospital of Henan Province, Henan 453000, PR China
2 Department of Endoscopy, Xinxiang Central Hospital of Henan Province, Henan 453000, PR China
3 Department of Oncology, 1st Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China

Correspondence Address:
Tian Guo-Fang
Department of Oncology, Xinxiang Central Hospital of Henan Province, Xinxiang, Henan 453000
PR China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.191615

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Aim: The aim of the study is to investigate the underlying molecular mechanisms by which gemcitabine (gem) inhibits proliferation and induces apoptosis in human pancreatic cancer PANC-1 cells in vitro. Materials and Methods: After PANC-1 cells had been treated by indicated concentration (0, 5, and 25 mg/L) of gem for 48 h, cell proliferation was evaluated by 3'-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay; cell morphology was observed by transmission electron microscopy; Expression of c-IAP2 and Bcl-2 proteins was analyzed by Western blot; the activity of caspase-3 and -9 was detected by spectrophotometry. Results: Gem significantly inhibited cell proliferation and could induce apoptosis of human pancreatic cancer PANC-1 cells, with a dose-dependent manner. Western blot analysis showed that gem significantly reduced c-IAP2 and Bcl-2 proteins expression level (P < 0.05). Spectrophotometric assay showed that gem significantly increased caspase-3 and -9 activity in PANC-1 cells. Conclusion: Gem could induce apoptosis of human pancreatic cancer PANC-1 cells, probably through downregulating c-IAP2 and Bcl-2 expression levels, and at the same time activating caspase-3 and -9.


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