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Year : 2016  |  Volume : 12  |  Issue : 1  |  Page : 379-385

Evaluation of transforming growth factor beta1 gene in oral submucous fibrosis induced in Sprague-Dawley rats by injections of areca nut and pan masala (commercial areca nut product) extracts

Department of Oral and Maxillofacial Pathology, Dr. Syamala Reddy Dental College, Hospital and Research Centre, Bangalore, Karnataka, India

Correspondence Address:
Venkatesh V Kamath
Department of Oral and Maxillofacial Pathology, Dr. Syamala Reddy Dental College, Hospital and Research Centre, Bangalore - 560 037, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1482.148729

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Context: Oral submucous fibrosis (OSF) is a potentially malignant oral disorder causally linked to the habit of chewing arecanut. The pathogenesis of the disorder is multifactorial and transforming growth factor beta (TGF-beta) is a prominent player in the induction of fibrosis. The alkaloids of the arecanut seem to target the TGF-beta and the deposition of collagen is predominantly mediated through this cytokine. Aims and Objectives: The present study attempts to induce OSF in Sprague-Dawley rats by injections with solutions of arecanut and pan masala extracts. The tissues were then analysed for the TGF-beta1 gene by real time polymerase chain reaction. (rtPCR). Materials and Methods: Buccal mucosa of Sprague-Dawley rats were injectedwith arecanut and pan masala solutions on alternate days over a period of 48. weeks. Quantitative real time PCR was done to assess the expression of TGF-beta1 in the tissues. Results: OSF-like lesions were seen in both the arecanut and pan masala.treated groups. The histological changes included atrophic epithelium, partial or complete loss of rete ridges, juxta-epithelial hyalinization, inflammation and accumulation of dense bundles of collagen fibers subepithelially. Quantitative real-time PCR showed a significant upregulation of TGF beta1. A. peak fold change of 4.74 in the 18th. week was observed for the arecanut group while the pan masala group recorded a peak change of 4.9 in the 24th. week. Conclusion: The study provides further evidence that arecanut and pan masala induce oral submucous fibrosisvia the TGF beta1 pathway.

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