Quantitative analysis of agnor counts of buccal mucosal cells of chewers and non chewers of gutkha: A comparative cytologic study

Nikhil I Malgaonkar; Kapil Dagrus; Srinivas S Vanaki; Rudrayya S Puranik; Manjunatha Bhari Sharanesha; Bassel Tarakji

doi:10.4103/0973-1482.148713

ORIGINAL ARTICLE

Year : 2016 | Volume : 12 | Issue : 1 | Page : 228-231

Quantitative analysis of agnor counts of buccal mucosal cells of chewers and non chewers of gutkha: A comparative cytologic study

Nikhil I Malgaonkar¹, Kapil Dagrus², Srinivas S Vanaki³, Rudrayya S Puranik³, Manjunatha Bhari Sharanesha⁴, Bassel Tarakji⁵
¹ Department of Oral Pathology, Yogita Dental College and Hospital, Khed, Maharashtra, India
² Department of Oral Pathology, K. M. Shah Dental College, Sumandeep Vidyapeeth University Campus, Vadodara, Gujarat, India
³ Department of Oral Pathology and Microbiology, P M Nadagowda Memorial Dental College and Hospital, Bagalkot, Karnataka, India
⁴ Department of Basic Dental Sciences, College of Dentistry, Al-Hawaiya, Taif University, Taif, Kingdom of Saudi Arabia
⁵ Department of Oral and Maxillofacial Sciences, Alfarabi College of Dentistry, Riyadh, Kingdom of Saudi Arabia

Correspondence Address:
Nikhil I Malgaonkar
Block num. 18, 3^rd Floor, ‘Laxmi Prasad’, Khandkar Lane, Tilak Nagar, Dombivli (East), Thane - 421 201, Maharashtra
India

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/0973-1482.148713

Aims and Objectives: The present study was taken up to evaluate the AgNOR counts in the buccal mucosa cells of gutkha chewers and compare that with the sex-matched controls. Materials and Methods: In all, 100 gutkha chewers and 50 sex-matched non-chewers (controls) were chosen. None of the patients in both groups had any clinical oral lesions or systemic diseases. After rinsing with 0.9% sodium chloride, cytologic smears were prepared and stained using the AgNOR method and observed in immersion oil at 1000 × magnification. Finally, 50 cells were selected at random; AgNOR dots were counted and their mean was recorded. The student t-test was used for analysis of data. Results: Comparison between mean AgNOR counts of gutkha chewers (2.68 ± 0.23) and non-chewers (2.01 ± 0.14) was found to be statistically significant. Conclusion: Cytology associated with AgNOR staining can effectively detect the early molecular changes within buccal mucosa cells of oral mucosa.

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