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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 4  |  Page : 1024-1029

Cyclo-oxygenase-2 expression in oral squamous cell carcinoma


1 Department of Oral and Maxillofacial Pathology of Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran
2 Department of Pathology of Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran
3 Department of Pathology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
4 Students Research Committee, Babol University of Medical Sciences, Babol, Iran
5 Non communicable Pediatrics Diseases Research Center, Babol University of Medical Sciences, Babol, Iran

Correspondence Address:
Maryam Seyedmajidi
Department of Oral and Maxillofacial Pathology, Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.138205

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Background: Many evidences showed that cyclooxygenase-2 (COX-2), an enzyme that catalyzes the synthesis of prostaglandins, has an important role in carcinogenesis. It has been observed in experimental models that selective COX-2 inhibitors suppress the formation of tumors including tongue carcinoma. The aim of this study was to investigate the immunohistochemical expression of COX-2 in oral squamous cell carcinoma (OSCC) compared to normal oral mucosa and oral dysplasia. Materials and Methods: A total of 60 paraffined blocks (20 cases of OSCC, 20 cases of oral epithelial dysplasia and 20 cases of normal oral mucosa were included in this study and immunohistochemical staining was done for COX-2 expression. From each sample, 5 high power fields were assessed to determine the percentage of stained cells and staining intensity. Immunoreactivity was obtained by multiplying the above two cases. Data were analyzed with using the Kruskal-Wallis test ANOVA, Mann-Whitney test and least significant difference and P < 0.05 was declared as significant. Results: High level of COX-2 expression was found in OSCC and dysplasia compared to normal mucosa. Furthermore, a positive correlation was found between COX-2 expression and severity of dysplasia. However, no significant difference between low grade and high grade tumors was found. Conclusion: The result of the present study supports from the role of COX-2 in carcinogenesis and progression of premalignant lesion to malignancy.

Abstract in Chinese

口腔鳞状细胞癌环氧合酶2的表达 摘要 背景:许多研究表明,环氧化酶2(COX 2),催化前列腺素合成的酶,在肿瘤发生中具有重要作用。实验模型中已经观察到选择性COX 2抑制剂抑制肿瘤的形成,包括舌癌。本研究的目的是比较口腔鳞状细胞癌(OSCC)组织正常口腔黏膜和口腔异型增生中COX 2的免疫组化表达。 材料与方法:共60个蜡块(20例口腔鳞癌,20例口腔上皮异常增生,20例正常口腔黏膜)。对COX 2表达做了免疫组化染色。从每个样品中,在5个高倍视野下评估,以确定染色细胞的百分比和染色强度。免疫反应性是上述两因素相乘。数据采用Kruskal-Wallis检验,方差分析,秩和检验和起码的显著差异,P<0.05为显著。 结果:在口腔鳞状细胞癌和异型增生中,COX 2表达水平高于正常黏膜。此外,发现COX 2表达与不典型增生的严重程度有正相关关系。然而,发现低级别和高级别肿瘤之间无显著性差异。 结论:本研究的结果支持COX 2的致癌作用及从癌前病变进展为恶性肿瘤中的作用。 关键词:环氧化酶2,不典型增生,免疫组织化学,正常口腔黏膜,鳞状细胞癌



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