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Year : 2014  |  Volume : 10  |  Issue : 2  |  Page : 312-316

Detection of survivin 2α gene expression in thyroid nodules

1 Department of Genetics, School of Medicine, Islamic Azad University, Najafabad Branch, Isfahan, Iran
2 Department of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran
3 Department of Nanobiomedicine, Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Pathology, Imam Khomeini Hospital, Tabriz University of Medical Sciences, Tabriz, Iran

Correspondence Address:
Mohammad Ali Hosseinpour Feizi
Department of Animal Biology, School of Natural Sciences, University of Tabriz, P.O. Box 51666-16471, Tabriz
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1482.136598

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Context: Functional studies of the survivin splice variants have been performed almost exclusively in various types of cancer and produced remarkable advances in our understanding of cancer biology and cancer genetics. Aim: To observation the expression of survivin 2α in thyroid nodules and estimate its potential as a new molecular marker in thyroid nodules screening and malignant thyroid, as well. Setting and Design: We detected the expression of a splice variant of survivin, survivin 2α, in thyroid nodules. Materials and Methods: Expression of survivin 2α mRNA was evaluated with specific primers by Hemi-Nested RT-PCR in 77 thyroid nodules including malignant and benign tumors, non-tumoral (goiter and thyroiditis) as well as surgical margin, non-neoplastic normal tissues adjacent to the malignant lesions. Result: Our data revealed for the first time the expression of survivin 2α in thyroid nodules. It was detected in 85.7% of non-neoplastic surgical margin tissues, 71.4% of non tumoral, 63.2% of tumoral samples. Also, the expression of survivin 2α in benign tumor samples (64.2%) is more than malignant groups (62.8%). Conclusion: Survivin 2α expression is the highest in non-neoplastic surgical margin rather than other samples and the lowest expression was that of malignancy. According to the results, it can be concluded that survivin 2α protein may be has a vital protective effect throw survivin quenching due to the high expression in normal tissue compared with lesions.

Abstract in Chinese

检测甲状腺结节中2αSurvivin基因的表达水平 摘要 背景:survivin拼接变异体的功能研究已在不同类型的肿瘤中都有涉及,它有助于了解癌症生物学和癌症遗传学方面有显著的进展。 目的:观察2αSurvivin基因在甲状腺结节表达,估计其成为甲状腺结节和甲状腺恶性肿瘤筛查的新的分子标记的潜力。 材料和方法:采用半巢式RT-PCR引物对2αSurvivin mRNA的表达进行了评估,分别取自 77个甲状腺结节,包括良性和恶性肿瘤,非肿瘤(甲状腺肿和甲状腺炎)以及手术切缘,非肿瘤旁正常组织的恶性病变。检测survivin剪接变异体及2αSurvivin基因在甲状腺结节中的表达。 结果:本研究发现甲状腺结节2非肿瘤手术切缘组织中αSurvivin阳性表达率85.7%,非肿瘤为71.4%,肿瘤组织为63.2%。2αSurvivin在良性肿瘤样本的阳性表达率(64.2%)高于恶性组(62.8%)。 结论:2αSurvivin在非肿瘤的手术切缘表达最高,在恶性肿瘤中表达最低。根据研究结果,可以得出结论,2αSurvivin蛋白可能具有重要的保护作用,正常组织较病变组织其表达程度更高。 关键词:半巢式RT PCR,剪接变异体,2αsurvivin基因,甲状腺癌

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