Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 176-179

Absence of correlation between serum CRP levels and mitochondrial D-loop DNA mutations in gastro-oesophageal adenocarcinoma

1 Clinical and Surgical Sciences (Surgery), University of Edinburgh, Royal Infirmary, Edinburgh, United Kingdom
2 Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, United Kingdom

Correspondence Address:
Benjamin H. L. Tan
University of Edinburgh, Tissue Injury and Repair Group, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB
United Kingdom
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1482.131458

Rights and Permissions

Introduction: Both inflammation and mitochondrial DNA (mtDNA) mutation are thought to play a role in the many human cancers. The aim of this study was to evaluate the relationship between inflammation and accumulation of mitochondrial DNA (mtDNA) mutations in the D-loop region in carcinogenesis of gastro-oesophageal adenocarcinomas. Materials and Methods: Blood samples of 20 patients with gastro-oesophageal adenocarcinoma were taken for measurement of serum C-reactive protein (CRP) concentration. Direct sequencing of mtDNA in the D-loop region was done in the 20 adenocarcinoma samples and their corresponding surrounding non-cancerous tissue. Sequences were compared with existing mtDNA databases to identify mutations. Results: mtDNA mutations in the D-loop region occur commonly with almost identical frequency in both non-cancerous tissue (3.0 ΁ 1.6) and adenocarcinoma (3.1 ΁ 1.9) (P = 0.916, paired t-test). CRP levels are not predictive of the number of D-loop mutations in both adenocarcinoma (β: -0.131; 95% CI: -2.354-1.364; P = 0.583) and non-cancerous tissue samples (β: 0.130; 95% CI: -1.125-1.933; P = 0.586). Five new mutations were identified that were not recorded previously in mtDNA databases. Conclusion: D-loop mtDNA mutations are common in both gastro-oesophageal adenocarcinoma and surrounding non-cancerous tissue. However, the accumulation of such mutations appears to occur independent of systemic inflammation. The frequency of D-loop mutations is likely not useful as a marker for carcinogenesis in gastro-oesophageal adenocarcinoma.

Abstract in Chinese

胃食管腺癌的血清CRP水平和线粒体D环的DNA突变之间无相关性 引言:炎症和线粒体DNA(mtDNA)突变被认为在许多人类癌症中起作用。本研究的目的是评估炎症和积累的线粒体DNA(mtDNA)D环区突变在胃食管腺癌癌变的关系。 材料和方法:对胃食管腺癌20例血液样本进行血清C-反应蛋白(CRP)浓度测定。对20例腺癌标本及其周围的非癌组织的D环区的线粒体DNA直接测序。与现有的mtDNA序列进行比较以确定突变数据。 结果:在D环区的mtDNA突变频率在非癌组织(3±1.6)和腺癌(3.1±1.9)中几乎相同(P = 0.916,配对t检验)。CRP水平不能预测D环突变数目,无论在腺癌(β:-0.131;95%可信区间:-2.354~1.364;P = 0.583),还是在非癌组织标本(β:0.130;95%可信区间:-1.125~1.933;P = 0.586)。有5个新的突变发现,先前并没有记录在线粒体DNA数据库。 结论:D环的线粒体DNA突变在胃食管腺癌和周围的非癌组织是常见的。然而,这种突变的积累似乎与不依赖全身性炎症的发生,D环突变的频率可能与胃食管腺癌的发生无相关性。 关键词:D环,胃食管腺癌,炎症,线粒体DNA突变,血清CRP

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded63    
    Comments [Add]    

Recommend this journal