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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 176-179

Absence of correlation between serum CRP levels and mitochondrial D-loop DNA mutations in gastro-oesophageal adenocarcinoma


1 Clinical and Surgical Sciences (Surgery), University of Edinburgh, Royal Infirmary, Edinburgh, United Kingdom
2 Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, United Kingdom

Correspondence Address:
Benjamin H. L. Tan
University of Edinburgh, Tissue Injury and Repair Group, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB
United Kingdom
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.131458

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Introduction: Both inflammation and mitochondrial DNA (mtDNA) mutation are thought to play a role in the many human cancers. The aim of this study was to evaluate the relationship between inflammation and accumulation of mitochondrial DNA (mtDNA) mutations in the D-loop region in carcinogenesis of gastro-oesophageal adenocarcinomas. Materials and Methods: Blood samples of 20 patients with gastro-oesophageal adenocarcinoma were taken for measurement of serum C-reactive protein (CRP) concentration. Direct sequencing of mtDNA in the D-loop region was done in the 20 adenocarcinoma samples and their corresponding surrounding non-cancerous tissue. Sequences were compared with existing mtDNA databases to identify mutations. Results: mtDNA mutations in the D-loop region occur commonly with almost identical frequency in both non-cancerous tissue (3.0 ΁ 1.6) and adenocarcinoma (3.1 ΁ 1.9) (P = 0.916, paired t-test). CRP levels are not predictive of the number of D-loop mutations in both adenocarcinoma (β: -0.131; 95% CI: -2.354-1.364; P = 0.583) and non-cancerous tissue samples (β: 0.130; 95% CI: -1.125-1.933; P = 0.586). Five new mutations were identified that were not recorded previously in mtDNA databases. Conclusion: D-loop mtDNA mutations are common in both gastro-oesophageal adenocarcinoma and surrounding non-cancerous tissue. However, the accumulation of such mutations appears to occur independent of systemic inflammation. The frequency of D-loop mutations is likely not useful as a marker for carcinogenesis in gastro-oesophageal adenocarcinoma.

Abstract in Chinese

胃食管腺癌的血清CRP水平和线粒体D环的DNA突变之间无相关性 引言:炎症和线粒体DNA(mtDNA)突变被认为在许多人类癌症中起作用。本研究的目的是评估炎症和积累的线粒体DNA(mtDNA)D环区突变在胃食管腺癌癌变的关系。 材料和方法:对胃食管腺癌20例血液样本进行血清C-反应蛋白(CRP)浓度测定。对20例腺癌标本及其周围的非癌组织的D环区的线粒体DNA直接测序。与现有的mtDNA序列进行比较以确定突变数据。 结果:在D环区的mtDNA突变频率在非癌组织(3±1.6)和腺癌(3.1±1.9)中几乎相同(P = 0.916,配对t检验)。CRP水平不能预测D环突变数目,无论在腺癌(β:-0.131;95%可信区间:-2.354~1.364;P = 0.583),还是在非癌组织标本(β:0.130;95%可信区间:-1.125~1.933;P = 0.586)。有5个新的突变发现,先前并没有记录在线粒体DNA数据库。 结论:D环的线粒体DNA突变在胃食管腺癌和周围的非癌组织是常见的。然而,这种突变的积累似乎与不依赖全身性炎症的发生,D环突变的频率可能与胃食管腺癌的发生无相关性。 关键词:D环,胃食管腺癌,炎症,线粒体DNA突变,血清CRP



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