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Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 165-170

Assessment of cytogenic damage in the form of micronuclei in oral epithelial cells in patients using smokeless and smoked form of tobacco and non-tobacco users and its relevance for oral cancer

1 Department of Oral Medicine and Radiology, Dr. D. Y. Patil Dental College, Pimpri, Pune, Maharashtra, India
2 Department of Oral Pathology and Microbiology, Bharati Vidyapeeth University, Pune, Maharashtra, India

Correspondence Address:
Anagha A Motgi
Department of Oral Medicine and Radiology, Dr. D. Y. Patil Dental College, Pimpri, Pune, Maharashtra
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1482.131454

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Context: Early detection of cytological damages may help in reduction of morbidity and mortality in patients with oral cancer. Aims: (1) The primary aim of this study is to assess the cytogenic damage in the form of micronuclei (MN) in patients with smokeless and smoked tobacco using habit. (2) The secondary aim of this study is to compare the MN score in patients using tobacco and patients with no tobacco habit. (3) To find out incidence of MN according to duration and frequency of tobacco usage. Settings and Design: This is a clinical study. Materials and Methods: A total of 100 patients each with the habit of smokeless tobacco (SLT) chewing, smoked tobacco usage and with no habit were included in the study. Epithelial cell smears were prepared and slides were stained with Papanicolaou stain. Scoring of at least 1000 cells was done and a MN frequency score was assigned for exfoliated oral mucosal cells. Statistical Analysis: Analysis of variance and post hoc tests were used. Results: The difference between the total number of cells with MN was not appreciable between the smokeless and smoked tobacco groups, though the total number of MN was higher in subjects using SLT. Total number of cells with MN and the total number of MN were significantly lower in non-tobacco users when compared with tobacco users. There was very weak positive correlation between the total number of MN as per the duration and frequency of the tobacco habit. Conclusion: The use of smokeless and smoked tobacco are associated with cytotoxic and genotoxic effects. SLT seems to cause more damaging effects than the smoked form.

Abstract in Chinese

使用无烟烟草、有烟烟草患者及非吸烟者口腔上皮细胞微核细胞损伤评估及其与口腔癌相关性 背景:细胞损害的早期检测有助于口腔癌患者的发病率和死亡率的降低。 目的:(1)本研究的主要目的是评估使用无烟烟草、有烟烟草患者的微核(MN)细胞遗传学损伤。(2)本研究的第二个目的是在使用烟草和病人没有吸烟习惯的病人间比较MN得分。(3)找出烟草使用时间和频率与微核(损伤)的关联。 设置和设计:这是一个临床研究。 材料和方法:共100例分别为无烟烟草(SLT)吸食者,有烟烟草吸食者及不吸烟者被纳入研究。制备了上皮细胞涂片和切片与巴氏染色。至少选定了口腔脱落黏膜细胞的1000个细胞评分和微核损伤频率得分。 统计分析:方差分析和Post Hoc 检验。 结果:无烟烟草组和有烟组之间,微核损伤细胞总数的差异并不明显,虽然微核损伤总量在SLT组高。微核损伤细胞总数及微核损伤总量在非吸烟组要明显低于吸烟组。微核损伤总量在烟草习惯的持续时间和频率上有弱正相关。 结论:无烟烟草和有烟烟草的使用与细胞毒性和遗传毒性的影响相关。无烟烟草似乎比有烟烟草会导致更多的伤害。 关键词:微核,口腔癌,有烟烟草,无烟烟草

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