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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 107-111

The investigation of changes in proteins expression (Apolipoprotein A1 and albumin) in malignant astrocytoma brain tumor


1 Department of Genetics, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Correspondence Address:
Mehrdad Hashemi
Department of Genetics, Islamic Azad University, Tehran Medical Sciences Branch, Tehran - 19395/1495
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.131413

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Objective: Angiogenesis performs a critical role in the development of cancer. Angiogenesis research is a cutting-edge field in cancer research. Proteomics is a powerful tool in identifying multiple proteins that are altered following a neuropharacological intervention in a disease of the central nervous system. Diagnostic oncoproteomics is the application of proteomic techniques for the diagnosis of malignancies. Materials and Methods: We extracted proteins of tumor and normal brain tissues and then evaluated the protein purity by Bradford test and spectrophotometery method. In this study, we separated proteins by two-dimensional (2D) gel electrophoresis method and the spots were then analyzed and compared using statistical data and specific software, after providing three-dimensional images of spots alteration. Spots were identified by pH isoelectric, molecular weights, and data banks. Results: Simple statistical test were used to establish a putative hierarchy in which the change in protein level were ranked according a cutoff point with P < 0.05. Apolipoprotein A1 (apo A1) protein and albumin were consistently upregulated in astrocytoma brain tumors. Conclusion: The vascular microenvironment of glioma play a major role in determining the pathophysiological character is tics of the tumor. apo A1 and albumin are very significant due to their functional consequences in glioma tumor growth, migration and angiogenesis.

Abstract in Chinese

恶性星形细胞脑瘤中蛋白质表达(阿朴脂蛋白和白蛋白)改变的研究 目的:血管生成在肿瘤发展中起到关键性作用。血管生成的研究是肿瘤研究中的前沿领域。在中枢神经系统疾病中,蛋白质组学在确认复杂的被神经干预而改变的蛋白质是强有力的工具。诊断癌蛋白质组学是癌症诊断中蛋白质组学技术的应用。 材料和方法:我们提取了肿瘤和正常脑组织中的蛋白质,然后用布拉德福试验和分光光度计评价了它们的纯度。此研究中,我们通过二维凝胶电泳法分离蛋白质,通过三维影像技术转化,再用统计学方法和特定软件对蛋白谱分析比较。蛋白谱通过等电位pH,分子量和数据库辩认。 结果:简单的统计检验用来建立假设的蛋白质改变层次水平,去掉不可信区间P<0.05。阿朴脂蛋白A1(apo A1)和白蛋白在脑星形细胞瘤中总是呈正调节。 结论:神经胶质瘤的血管微环境在决定肿瘤的病理生理特性中起主要作用。阿朴脂蛋白A1和白蛋白在神经胶质瘤生长、转移和血管生成方面需要引起注意,它们在上述方面有较强功能。 关键词:白蛋白二维凝胶电泳,星形细胞瘤,蛋白质组学,阿朴脂蛋白A1



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