Journal of Cancer Research and Therapeutics Close
 

Figure 2: CD44+/CD24 cells were treated with Notch1 inhibitor DAPT. (a) Expression of Notch1 mRNA in CD44+/CD24 cells treated with DAPT in different concentrations (0, 25, 50, and 75 μM) for 72 h was detected by polymerase chain reaction. (b) Expression of Notch1 protein in CD44+/CD24 cells treated with DAPT in different concentrations (0, 25, 50, and 75 μM) for 72 h was detected by western blot. (c) Proliferation of CD44+/CD24 cells after treatment with 50 μM DAPT for 2 weeks was detected by colony-forming assay

Figure 2: CD44<sup>+</sup>/CD24<sup>−</sup> cells were treated with Notch1 inhibitor DAPT. (a) Expression of Notch1 mRNA in CD44<sup>+</sup>/CD24<sup>−</sup> cells treated with DAPT in different concentrations (0, 25, 50, and 75 μM) for 72 h was detected by polymerase chain reaction. (b) Expression of Notch1 protein in CD44<sup>+</sup>/CD24<sup>−</sup> cells treated with DAPT in different concentrations (0, 25, 50, and 75 μM) for 72 h was detected by western blot. (c) Proliferation of CD44<sup>+</sup>/CD24<sup>−</sup> cells after treatment with 50 μM DAPT for 2 weeks was detected by colony-forming assay