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Figure 1: Isolation and identification of CD44+/CD24 cells from A549 cells and the detection of Notch1 expression. (a) Spheres formed by isolated CD44+/CD24 cells under serum-free culture conditions. (b) Expression of cancer stem cells marker ALDH1A in CD44+/CD24 cells and unsorted A549 cells was detected by immunocytochemistry. (c) Expression of Notch1 mRNA in CD44+/CD24 cells and unsorted A549 cells was detected by polymerase chain reaction. (d) Expression of Notch1 protein in CD44+/CD24 cells and unsorted A549 cells was detected by western blot

Figure 1: Isolation and identification of CD44<sup>+</sup>/CD24<sup>−</sup> cells from A549 cells and the detection of Notch1 expression. (a) Spheres formed by isolated CD44<sup>+</sup>/CD24<sup>−</sup> cells under serum-free culture conditions. (b) Expression of cancer stem cells marker ALDH1A in CD44<sup>+</sup>/CD24<sup>−</sup> cells and unsorted A549 cells was detected by immunocytochemistry. (c) Expression of Notch1 mRNA in CD44<sup>+</sup>/CD24<sup>−</sup> cells and unsorted A549 cells was detected by polymerase chain reaction. (d) Expression of Notch1 protein in CD44<sup>+</sup>/CD24<sup>−</sup> cells and unsorted A549 cells was detected by western blot