Journal of Cancer Research and Therapeutics Close
 

Figure 1: Effect of Wnt5a overexpression on the cell cycle progression, colony‑forming potential, and motility of SMMC‑7721 cells. (a) Expression of Wnt5a in SMMC‑7721 cells. The overexpression of Wnt5a in SMMC‑7721/Wnt5a cells indicated successful transfection. ƒÀ‑actin detection confirmed equal loading. (Wnt5: SMMC‑7721/Wnt5a; Control: SMMC‑7721/pcDNA3.1). (b) Flow cytometric analysis of cell cycle progression. (c) Effect of Wnt5a on clonogenicity of SMMC‑7721 cells. SMMC‑7721/Wnt5a cells displayed lower clonogenicity than that of SMMC‑7721/ pcDNA3.1 cells after 25 days of culture. (d) The motility of SMMC‑7721/pcDNA3.1 and SMMC‑7721/Wnt5a cells was determined by the wound migration assay. The spreading of SMMC‑7721/Wnt5a cells along the edges of the wound was significantly decreased compared with those that of SMMC‑7721/pcDNA3.1 cells after 48 h

Figure 1: Effect of Wnt5a overexpression on the cell cycle progression, colony‑forming potential, and motility of SMMC‑7721 cells. (a) Expression of Wnt5a in SMMC‑7721 cells. The overexpression of Wnt5a in SMMC‑7721/Wnt5a cells indicated successful transfection. ƒÀ‑actin detection confirmed equal loading. (Wnt5: SMMC‑7721/Wnt5a; Control: SMMC‑7721/pcDNA3.1). (b) Flow cytometric analysis of cell cycle progression. (c) Effect of Wnt5a on clonogenicity of SMMC‑7721 cells. SMMC‑7721/Wnt5a cells displayed lower clonogenicity than that of SMMC‑7721/ pcDNA3.1 cells after 25 days of culture. (d) The motility of SMMC‑7721/pcDNA3.1 and SMMC‑7721/Wnt5a cells was determined by the wound migration assay. The spreading of SMMC‑7721/Wnt5a cells along the edges of the wound was significantly decreased compared with those that of SMMC‑7721/pcDNA3.1 cells after 48 h