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Figure 3: Methylation-specific polymerase chain reaction analysis for the methylation of RUNX3 (a) and RASSF1A (b) in serum. Genomic DNA from b solitary pulmonary nodule tissues treated with sodium bisulfite was amplified using methylated (M) and unmethylated primers (U). P was used as a positive control for methylated reaction, and N was used as a positive control for unmethylated reaction. Ma denotes malignant solitary pulmonary nodule and Be denotes benign solitary pulmonary nodule

Figure 3: Methylation-specific polymerase chain reaction analysis for the methylation of RUNX3 (a) and RASSF1A (b) in serum. Genomic DNA from b solitary pulmonary nodule tissues treated with sodium bisulfite was amplified using methylated (M) and unmethylated primers (U). <i>P</i> was used as a positive control for methylated reaction, and N was used as a positive control for unmethylated reaction. Ma denotes malignant solitary pulmonary nodule and Be denotes benign solitary pulmonary nodule