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Figure 5: Effects of FL34 on biomarkers associated with proliferation, invasion, angiogenesis pathways, and the regulation of apoptosis in U-87 MG and T98G cells and U-87 MG xenograft tumors treated with FL34. Cells were treated with the indicated concentrations of FL34 for 72 h. “Con” refers to untreated control samples. Whole-cell lysates were subjected to western blotting with antibodies against the indicated proteins. Mice bearing U-87 MG tumors were randomized and treated with 15.0 or 30.0 mg/kg FL34 daily for 14 days. Lysates from two or three mice per group were pooled. Each lane represents one protein pool, and two or three pools/groups were subjected to western blot analysis. β-actin was included as a loading control. Representative western blot analysis results are presented for each experiment. (a) Western blot analysis of ERK and AKT in U-87 MG, T98G cells treated with FL34 for 72 h. (b) Western blot analysis of ERK and AKT in U-87 MG xenograft tumors treated with FL34. (c) Western blot analysis of regulators of apoptosis in U-87 MG and T98G cells treated with FL34 for 72 h. (d) Western blot analysis of regulators of apoptosis in U-87 MG xenograft tumors treated with FL34. (e) Western blot analysis of proteins related to invasion and angiogenesis in U-87 MG and T98G cells treated with FL34 for 72 h. (f) Western blot analysis of proteins related to invasion and angiogenesis in U-87 MG xenograft tumors treated with FL34

Figure 5: Effects of FL34 on biomarkers associated with proliferation, invasion, angiogenesis pathways, and the regulation of apoptosis in U-87 MG and T98G cells and U-87 MG xenograft tumors treated with FL34. Cells were treated with the indicated concentrations of FL34 for 72 h. “Con” refers to untreated control samples. Whole-cell lysates were subjected to western blotting with antibodies against the indicated proteins. Mice bearing U-87 MG tumors were randomized and treated with 15.0 or 30.0 mg/kg FL34 daily for 14 days. Lysates from two or three mice per group were pooled. Each lane represents one protein pool, and two or three pools/groups were subjected to western blot analysis. β-actin was included as a loading control. Representative western blot analysis results are presented for each experiment. (a) Western blot analysis of ERK and AKT in U-87 MG, T98G cells treated with FL34 for 72 h. (b) Western blot analysis of ERK and AKT in U-87 MG xenograft tumors treated with FL34. (c) Western blot analysis of regulators of apoptosis in U-87 MG and T98G cells treated with FL34 for 72 h. (d) Western blot analysis of regulators of apoptosis in U-87 MG xenograft tumors treated with FL34. (e) Western blot analysis of proteins related to invasion and angiogenesis in U-87 MG and T98G cells treated with FL34 for 72 h. (f) Western blot analysis of proteins related to invasion and angiogenesis in U-87 MG xenograft tumors treated with FL34