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Figure 7: Temozolomide treatment and Tim-3 siRNA induce apoptotic cell death. Dot-plot analysis from flow cytometry in 5% fetal bovine serum medium or serum-free medium. Percentage of cells according to viability and type of cell death: PE/7AAD− (viable cells), PE+/7AAD+ (late apoptosis), PE+/7AAD− (early apoptosis), and PE−/7AAD+ (necrosis). Data are represented as mean ± standard error of the mean (n = 6). *P < 0.05 versus control, #P < 0.05 versus 1200 μM temozolomide group

Figure 7: Temozolomide treatment and Tim-3 siRNA induce apoptotic cell death. Dot-plot analysis from flow cytometry in 5% fetal bovine serum medium or serum-free medium. Percentage of cells according to viability and type of cell death: PE/7AAD− (viable cells), PE+/7AAD+ (late apoptosis), PE+/7AAD− (early apoptosis), and PE−/7AAD+ (necrosis). Data are represented as mean ± standard error of the mean (<i>n</i> = 6). *<i>P</i> < 0.05 versus control, <sup>#</sup><i>P</i> < 0.05 versus 1200 μM temozolomide group