Journal of Cancer Research and Therapeutics Close
 

Figure 8: Summary and workflow of the study: Out of 104 histopathologically confirmed cases of esophageal cancer, 90 esophageal squamous cell carcinoma patients were selected for investigation. Tumor suppressor genes methylation profiling was done in 6 paired Grade 2 tumor and their adjacent normal tissues. Peak hypermethylation was found in OPCML, hence its expression was examined by tissue microarray-based immunohistochemistry. No significant change in OPCML expression among control, Grade 1, and Grade 2 tumor was found. Therefore, no effect on gene expression is manifested by hypermethylated promoter in question. However, protein expression of OPCML was found sharply decreased in Grade 3 compared to Grade 2, Grade 1 tumor and control tissues suggesting its association with early carcinogenesis episode (Differential OPCML expression represented in the figure is not scale based). Twenty three genes identified in Integrome network analysis in a previous genome-wide methylation study from the same population along with eight tumor suppressor genes from the present investigation were subjected to protein–protein interaction analysis by STRING. Gene enrichment revealed significant Biological Processes (GO) as cellular response to nitrogen compound (GO: 1901699) and cellular responses to organic cyclic compound (GO: 0071407). Deregulation of Wnt/β-catenin and TGF-β signaling pathways is revealed in the present study, which mediates by epigenetic alterations in CDH1, WT1, THBS1, TERT, PTK2, CTNNB1, and CTNND2 genes and also by distorted OPCML protein expression

Figure 8: Summary and workflow of the study: Out of 104 histopathologically confirmed cases of esophageal cancer, 90 esophageal squamous cell carcinoma patients were selected for investigation. Tumor suppressor genes methylation profiling was done in 6 paired Grade 2 tumor and their adjacent normal tissues. Peak hypermethylation was found in OPCML, hence its expression was examined by tissue microarray-based immunohistochemistry. No significant change in OPCML expression among control, Grade 1, and Grade 2 tumor was found. Therefore, no effect on gene expression is manifested by hypermethylated promoter in question. However, protein expression of OPCML was found sharply decreased in Grade 3 compared to Grade 2, Grade 1 tumor and control tissues suggesting its association with early carcinogenesis episode (Differential OPCML expression represented in the figure is not scale based). Twenty three genes identified in Integrome network analysis in a previous genome-wide methylation study from the same population along with eight tumor suppressor genes from the present investigation were subjected to protein–protein interaction analysis by STRING. Gene enrichment revealed significant Biological Processes (GO) as cellular response to nitrogen compound (GO: 1901699) and cellular responses to organic cyclic compound (GO: 0071407). Deregulation of Wnt/β-catenin and TGF-β signaling pathways is revealed in the present study, which mediates by epigenetic alterations in CDH1, WT1, THBS1, TERT, PTK2, CTNNB1, and CTNND2 genes and also by distorted OPCML protein expression