Journal of Cancer Research and Therapeutics Close
 

Figure 4: Inhibition of the activity of gamma-secretase by difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester (gamma-secretase inhibitor) diminishes tumor growth. (a) The activities of gamma-cleavage in difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (a) and HepG2 (e) cells were measured using a luciferase-based gamma-secretase reporter system. Data are presented as percent activity where 100% activity corresponds to the reporter gene activity obtained in the vehicle control. (b) Cell viabilities of difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (b) and HepG2 cells (f) were assayed for consecutive 5 days using the cell counting kit 8 methods. (c) Difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (c) and HepG2 cells (g) cells were plated in six-well plates for soft agar colony assay, and experiments were performed in duplicates. Representative data of one experiment is shown. Data are presented as percent value where 100% value corresponds to the colony obtained in the vehicle control. (d) Difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (d) and HepG2 cells (h) cells were plated on collagen-precoated plates for cell adhesion assay. Data are presented as percent value where 100% value corresponds to the order deviation value obtained in the vehicle control. *P < 0.05; **P < 0.01

Figure 4: Inhibition of the activity of gamma-secretase by difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester (gamma-secretase inhibitor) diminishes tumor growth. (a) The activities of gamma-cleavage in difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (a) and HepG2 (e) cells were measured using a luciferase-based gamma-secretase reporter system. Data are presented as percent activity where 100% activity corresponds to the reporter gene activity obtained in the vehicle control. (b) Cell viabilities of difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (b) and HepG2 cells (f) were assayed for consecutive 5 days using the cell counting kit 8 methods. (c) Difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (c) and HepG2 cells (g) cells were plated in six-well plates for soft agar colony assay, and experiments were performed in duplicates. Representative data of one experiment is shown. Data are presented as percent value where 100% value corresponds to the colony obtained in the vehicle control. (d) Difluorophenylacetyl-alanyl-phenylglycine-t-butyl-ester treated BEL7404 (d) and HepG2 cells (h) cells were plated on collagen-precoated plates for cell adhesion assay. Data are presented as percent value where 100% value corresponds to the order deviation value obtained in the vehicle control. *<i>P</i> < 0.05; **<i>P</i> < 0.01