Journal of Cancer Research and Therapeutics Close
 

Figure 1: CD13 and CD34 are cancer stem cell markers for H1650 cell lines. (a) H1650 cell lines were labeled with Hoechst 33342 dye and analyzed by flow cytometry before and after treatment with verapamil. (b) Relative expression of stem cell markers in side population and nonside population cells. Data are presented as the mean ± standard deviation (n = 3), **P < 0.005. (c) Relative expression of stem cell markers in parental cells and spheres. Data are presented as the mean ± standard deviation (n = 3), **P < 0.005, ***P < 0.001. (d) CD133+ CD34 and CD133 CD34+ cells were treated with the indicated concentrations of doxorubicin for 24 h.. Concentrations of doxorubicin for 24 h. Cell lysates were analyzed by Western blots with poly ADP-ribose polymerase and tubulin antibodies. (e) CD133+ CD34 and CD133 CD34+ cells were treated with the indicated intensities of γ-irradiation. Cell lysates were analyzed by Western blots with poly ADP-ribose polymerase and tubulin antibodies

Figure 1: CD13 and CD34 are cancer stem cell markers for H1650 cell lines. (a) H1650 cell lines were labeled with Hoechst 33342 dye and analyzed by flow cytometry before and after treatment with verapamil. (b) Relative expression of stem cell markers in side population and nonside population cells. Data are presented as the mean ± standard deviation (<i>n</i> = 3), **<i>P</i> < 0.005. (c) Relative expression of stem cell markers in parental cells and spheres. Data are presented as the mean ± standard deviation (<i>n</i> = 3), **<i>P</i> < 0.005, ***<i>P</i> < 0.001. (d) CD133<sup>+</sup> CD34<sup>−</sup> and CD133<sup>−</sup> CD34<sup>+</sup> cells were treated with the indicated concentrations of doxorubicin for 24 h.. Concentrations of doxorubicin for 24 h. Cell lysates were analyzed by Western blots with poly ADP-ribose polymerase and tubulin antibodies. (e) CD133<sup>+</sup> CD34<sup>−</sup> and CD133<sup>−</sup> CD34<sup>+</sup> cells were treated with the indicated intensities of γ-irradiation. Cell lysates were analyzed by Western blots with poly ADP-ribose polymerase and tubulin antibodies