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Figure 3: Western blot analysis for the relative protein levels of JMJD5 in MCF-7 and MDA-MB-231 cells. (a) The effects of different concentration of soybean on relative protein levels of JMJD5 in MCF-7 cells. (b) Relative protein levels of JMJD5 in the transfected or nontransfected MCF-7 cells. (c) The effects of different concentration of soybean on protein levels of JMJD5 in MDA-MB-231 cells. (d) The relative protein levels of JMJD5 in the transfected or nontransfected MDA-MB-231 cells. Control, MCF-7, and MDA-MB-231 cells were not transfected with any gene. Control + COS, MCF-7, and MDA-MB-231 cells were treated with 2 mg/ml COS. JMJD5, the cells were transfected with pcDNA-3.1-JMJD5. JMJD5 + COS, the cells were transfected with pcDNA-3.1-JMJD5 and treated with 2 mg/ml COS. JMJD5 shRNA, the cells were transfected with the vector pTZU6+1-shRNA-JMJD5. JMJD5 shRNA + COS, the cells were transfected with the vector pTZU6+1-shRNA-JMJD5 and treated with 2 mg/ml COS. JMJD5 shRNA. Each bar was presented as the mean values ± standard deviation of three independent experiments. *P < 0.05 via a control group. #P < 0.05 via the control without the addition of soybean peptides

Figure 3: Western blot analysis for the relative protein levels of JMJD5 in MCF-7 and MDA-MB-231 cells. (a) The effects of different concentration of soybean on relative protein levels of JMJD5 in MCF-7 cells. (b) Relative protein levels of JMJD5 in the transfected or nontransfected MCF-7 cells. (c) The effects of different concentration of soybean on protein levels of JMJD5 in MDA-MB-231 cells. (d) The relative protein levels of JMJD5 in the transfected or nontransfected MDA-MB-231 cells. Control, MCF-7, and MDA-MB-231 cells were not transfected with any gene. Control + COS, MCF-7, and MDA-MB-231 cells were treated with 2 mg/ml COS. JMJD5, the cells were transfected with pcDNA-3.1-JMJD5. JMJD5 + COS, the cells were transfected with pcDNA-3.1-JMJD5 and treated with 2 mg/ml COS. JMJD5 shRNA, the cells were transfected with the vector pTZU6+1-shRNA-JMJD5. JMJD5 shRNA + COS, the cells were transfected with the vector pTZU6+1-shRNA-JMJD5 and treated with 2 mg/ml COS. JMJD5 shRNA. Each bar was presented as the mean values ± standard deviation of three independent experiments. *<i>P</i> < 0.05 via a control group. <sup>#</sup><i>P</i> < 0.05 via the control without the addition of soybean peptides