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Figure 3: High content analysis of time- and dose-dependence of nuclear factor-kappa B activation by deoxycholic acid in esophageal cells. SKGT-4 cells (a and b) or OE33 cells (c and d) were treated with 300 μM deoxycholic deoxycholic for doses of acid acid different periods of time (0-4 h) or with different (100-400 μM) for 2 h and then stained for nuclear factor-kappa B. The difference in nuclear factor-kappa B fluorescence intensities between the nuclear and cytoplasmic regions of the same cells were measured and plotted. RingAvgInten, CircAvgInten and CircRingAvgIntenDiffCh2 in SKGT-4 cells or CircRingAvgIntenDiffCh2 in OE33 cells are shown

Figure 3: High content analysis of time- and dose-dependence of nuclear factor-kappa B activation by deoxycholic acid in esophageal cells. SKGT-4 cells (a and b) or OE33 cells (c and d) were treated with 300 μM deoxycholic deoxycholic for doses of acid acid different periods of time (0-4 h) or with different (100-400 μM) for 2 h and then stained for nuclear factor-kappa B. The difference in nuclear factor-kappa B fluorescence intensities between the nuclear and cytoplasmic regions of the same cells were measured and plotted. RingAvgInten, CircAvgInten and CircRingAvgIntenDiffCh2 in SKGT-4 cells or CircRingAvgIntenDiffCh2 in OE33 cells are shown