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Figure 1: Expression of miR-542-3p in esophageal squamous cell carcinoma samples and cell lines. (a) Relative expression of miR-542-3p in human esophageal squamous cell carcinoma samples and paired normal esophageal tissues. Quantitative real-time polymerase chain reaction was performed on 60 paired esophageal squamous cell carcinoma samples. The miR-542-3p expression levels were normalized to that of U6B. ΔCt(N): Ct value of miR-542-3p t value of U6B in normal tissue. ΔCt(T): Ct value of miR-542-3p t value of U6B in tumor tissue. Bar value ≤−1 indicates that the expression of miR-542-3p is down-regulated in tumors. Bar value ≥1 indicates that the expression of miR-542-3p is up-regulated in tumors. (b) Relative expression of miR-542-3p in human esophageal cells and esophageal squamous cell carcinoma cells. MiR-542-3p expression was detected in normal esophageal epithelial cell line (human esophageal epithelium cells) and six esophageal squamous cell carcinoma cell lines (Eca-109, Ec-9706, TE-1, TE-13, KYSE-150, and KYSE-410) by quantitative real-time polymerase chain reaction. U6B was used as internal control

Figure 1: Expression of miR-542-3p in esophageal squamous cell carcinoma samples and cell lines. (a) Relative expression of miR-542-3p in human esophageal squamous cell carcinoma samples and paired normal esophageal tissues. Quantitative real-time polymerase chain reaction was performed on 60 paired esophageal squamous cell carcinoma samples. The miR-542-3p expression levels were normalized to that of U6B. ΔCt(N): Ct value of miR-542-3p t value of U6B in normal tissue. ΔCt(T): Ct value of miR-542-3p t value of U6B in tumor tissue. Bar value ≤−1 indicates that the expression of miR-542-3p is down-regulated in tumors. Bar value ≥1 indicates that the expression of miR-542-3p is up-regulated in tumors. (b) Relative expression of miR-542-3p in human esophageal cells and esophageal squamous cell carcinoma cells. MiR-542-3p expression was detected in normal esophageal epithelial cell line (human esophageal epithelium cells) and six esophageal squamous cell carcinoma cell lines (Eca-109, Ec-9706, TE-1, TE-13, KYSE-150, and KYSE-410) by quantitative real-time polymerase chain reaction. U6B was used as internal control