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Figure 3: Effects of CME-1 on MMP-1 expression in B16F10 melanoma cell line. B16F10 (2×104) cells were treated with CME-1 (250, 500, and 800 g/ml) for 24 h. Cell lysates were obtained and analyzed for MMP- 1 protein expression by Western blotting (bottom, densitometric analysis of bands for MMP-1, relative to α-tubulin normalized to the normal condition). *P < 0.05 and, **P < 0.01 compared with the normal untreated B16 melanoma cells

Figure 3: Effects of CME-1 on MMP-1 expression in B16F10 melanoma cell line. B16F10 (2×104) cells were treated with CME-1 (250, 500, and 800 g/ml) for 24 h. Cell lysates were obtained and analyzed for MMP- 1 protein expression by Western blotting (bottom, densitometric analysis of bands for MMP-1, relative to α-tubulin normalized to the normal condition). *<i>P</i> < 0.05 and, **<i>P</i> < 0.01 compared with the normal untreated B16 melanoma cells