Figure 4: HF1 localization in Dalton's lymphoma (DL) cells. In all, 1.0 × 105 DL cells were treated with chelerythrine IC50 10μg/mL in a similar experimental condition as in previous experiments. DL cells were fixed on slides and incubated with mouse anti-HSF1 and then ALP conjugated secondary antibody. Then, the slides were incubated with substrate NBT/BCIP and color intensity was detected in terms of the number of purple granules under light microscope. Data are representative of three independent experiments. (a) Negative control (b) Positive control with H2O2, (c) chelerythrine treatment before heat shock, (d) chelerythrine treatment after heat shock.(e) 6-h chelerythrine treatment without heat shock, (f) 12-h chelerythrine treatment without heat shock.