Journal of Cancer Research and Therapeutics Close
 

Figure 3: Expression of HSF1 and hsp70 in Dalton's lymphoma cells. In all, 1.0 × 105 DL cells were treated with or without chelerythrine IC50 10 μg/mL in different experimental conditions and the expression of nHSF1 and cHSF1 and hsp70 was determined by Western blotting and RT-PCR. L1 shows negative control, L2 positive control with PMA, L3 chelerythrine before heat shock, L4 chelerythrine after heat shock, L5 chelerythrine 6 h without heat shock, L6 and chelerythrine 12 h without heat shock. Each data point is representative of at least three independent experiments. (a) Shows the Western blot of nHSF1, cHSF1, and hsp70 protein.(b) Shows the relative band intensity of blots; and (c) shows the RT-PCR analysis for the expression of HSF1 and hsp70

Figure 3: Expression of HSF1 and hsp70 in Dalton's lymphoma cells. In all, 1.0 × 10<sup>5</sup> DL cells were treated with or without chelerythrine IC<sub>50</sub> 10 μg/mL in different experimental conditions and the expression of nHSF1 and cHSF1 and hsp70 was determined by Western blotting and RT-PCR. L1 shows negative control, L2 positive control with PMA, L3 chelerythrine before heat shock, L4 chelerythrine after heat shock, L5 chelerythrine 6 h without heat shock, L6 and chelerythrine 12 h without heat shock. Each data point is representative of at least three independent experiments. (a) Shows the Western blot of nHSF1, cHSF1, and hsp70 protein.(b) Shows the relative band intensity of blots; and (c) shows the RT-PCR analysis for the expression of HSF1 and hsp70