Figure 2: Effect of chelerythrine on protein kinase C (PKC) activity. In all, 1.0 × 105 DL cells were treated with chelerythrine IC50 10μg/ mL under different experimental conditions as described. Dalton's lymphoma cells were lysed, centrifuged at 100,000×g, and relative kinase was determined in terms of ng/assay as described in the "Material and Methods" section. ((a) Indicates PKC activity in cytosolic fraction.(b) Indicates PKC activity in membrane bound fraction). Data are representative of three independent experiments.